Ads
related to: trizol protocol
Search results
Results From The WOW.Com Content Network
It was originally devised by Piotr Chomczynski and Nicoletta Sacchi, who published their protocol in 1987. [2] [3] The reagent is sold by Sigma-Aldrich by the name TRI Reagent; by Invitrogen under the name TRIzol; by Bioline as Trisure; and by Tel-Test as STAT-60.
TRIzol is a widely used [1] chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.
Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely.. Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them.
Aqueous samples, lysed cells, or homogenised tissue are mixed with equal volumes of a phenol:chloroform mixture. This mixture is then centrifuged. Because the phenol:chloroform mixture is immiscible with water, the centrifuge will cause two distinct phases to form: an upper aqueous phase, and a lower organic phase.
Nicoletta Sacchi (born August 20, 1949) is an Italian professor of oncology at the Roswell Park Comprehensive Cancer Center. [1] She is the co-discoverer of the acid guanidinium thiocyanate-phenol-chloroform extraction method to extract RNA from biological samples with Trizol. [2]
Witnesses to the New Year’s truck-ramming in New Orleans this week say barriers that could have prevented the suspect from driving down Bourbon Street were not deployed, …
Quantitation is made in reference to a methylated reference DNA. A modification to this protocol to increase the specificity of the PCR for successfully bisulfite-converted DNA (ConLight-MSP) uses an additional probe to bisulfite-unconverted DNA to quantify this non-specific amplification. [18]
Helicobacter pylori eradication protocols is a standard name for all treatment protocols for peptic ulcers and gastritis in the presence of Helicobacter pylori infection. The primary goal of the treatment is not only temporary relief of symptoms but also total elimination of H. pylori infection.
Ad
related to: trizol protocol