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The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). [1] Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid ...
It combines the functions of a thermal cycler and a fluorimeter, enabling the process of quantitative PCR. The first quantitative PCR machine was described in 1993, [2] and two commercial models became available in 1996. By 2009, eighteen different models were offered by seven different manufacturers. [3]
A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
Complex thermal cyclers were constructed to perform the Klenow-based amplifications, but never marketed. Simpler machines for Taq-based PCR were developed, and on November 19, 1987, a press release announces the commercial availability of the "PCR-1000 Thermal Cycler" and "AmpliTaq DNA Polymerase".
The particle densities tend to be in the range of 10 18 to 10 22 m −3 and the linear dimensions in the range of 0.1 to 10 m. The particle and energy confinement times may range from under a millisecond to over a second, but the configuration itself is often maintained through input of particles, energy, and current for times that are hundreds ...
The 3C-like protease (3CL pro) or main protease (M pro), formally known as C30 endopeptidase or 3-chymotrypsin-like protease, [2] is the main protease found in coronaviruses. It cleaves the coronavirus polyprotein at eleven conserved sites. It is a cysteine protease and a member of the PA clan of proteases.
However, water has a very high volumetric heat capacity, at 4.18 MJ⋅K −1 ⋅m −3, and ammonia is also fairly high: 3.3 MJ⋅K −1 ⋅m −3. For gases at room temperature, the range of volumetric heat capacities per atom (not per molecule) only varies between different gases by a small factor less than two, because every ideal gas has ...
A thermodynamic cycle consists of linked sequences of thermodynamic processes that involve transfer of heat and work into and out of the system, while varying pressure, temperature, and other state variables within the system, and that eventually returns the system to its initial state. [1]
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