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3.) Isoenzymes of alkaline phosphatase: [7] Six isoenzymes have been identified. The enzyme is a monomer, the isoenzymes are due to the differences in the carbohydrate content (sialic acid residues). The most important ALP isoenzymes are α 1-ALP, α 2-heat labile ALP, α 2-heat stable ALP, pre-β ALP and γ-ALP.
Aldolase B is a homotetrameric enzyme, composed of four subunits with molecular weights of 36 kDa with local 222 symmetry. Each subunit has a molecular weight of 36 kDa and contains an eight-stranded α/β barrel, which encloses lysine 229 (the Schiff-base forming amino acid that is key for catalysis).
The mechanism of ring closure is complex, but involves an aldol condensation at C-2 and C-7.. Metabolic engineering has improved production of DAHP by Escherichia coli. [1] The first step, condensation of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) from PEP/E4P, uses three isoenzymes AroF, AroG, and AroH. [2]
Hexokinases I, II, and III are referred to as low-K m isoenzymes because of a high affinity for glucose (below 1 mM). Hexokinases I and II follow Michaelis-Menten kinetics at physiological concentrations of substrates. [citation needed] All three are strongly inhibited by their product, glucose-6-phosphate. Molecular masses are around 100 kDa ...
As a result, the kinetic and regulatory properties of the various isoenzymes pools are dependent on subunit composition. Tissue-specific changes in PFK activity and isoenzymic content contribute significantly to the diversities of glycolytic and gluconeogenic rates which have been observed for different tissues. [4]
Glycogen phosphorylase isoenzyme BB: 0.854 and 0.767 [12] 7 hours Glycogen phosphorylase isoenzyme BB (abbreviation: GPBB) is one of the three isoforms of glycogen phosphorylase. This isoform of the enzyme exists in cardiac (heart) and brain tissue. Because of the blood–brain barrier, GP-BB can be seen as being specific to heart muscle.
In molecular biology, a multienzyme complex is a protein complex containing several copies of one or more enzymes packed into one macromolecular assembly.Multienzyme complexes carry out a single or multi-step biochemical reaction taking place within cells.
The study of enzymes is called enzymology and the field of pseudoenzyme analysis recognizes that during evolution, some enzymes have lost the ability to carry out biological catalysis, which is often reflected in their amino acid sequences and unusual 'pseudocatalytic' properties. [2] [3]