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Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid ...
A graduated pipette is a pipette with its volume, in increments, marked along the tube. It is used to accurately measure and transfer a volume of liquid from one container to another. [ 1 ] It is made from plastic or glass tubes and has a tapered tip.
These pipettes operate by piston-driven air displacement. A vacuum is generated by the vertical travel of a metallic or ceramic piston within an airtight sleeve. The upward movement of the piston, driven by the depression of the plunger, creates a vacuum in the space left vacant by the piston.
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette.The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [1] as it reduces the risk of splashing, foam or bubble formation.
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop. After about one second, keep on pressing pipette knob to the second stop. This action will completely empty the tip from the liquid. Afterwards withdraw the tip from the liquid and release the pipette to the ready position.
It serves as a vacuum source for filling reagents through a pipette or pasteur pipette and also help control the flow of liquid from the dropping bottle. [1] By using rubber bulb, the contact of the mouth to the chemicals can be avoided. These rubber rods come in different shapes, sizes and colors. [2]