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The most common applications are used as fluorescent probes, either directly or indirectly, such as aminoallyl nucleotide, which are used to label cRNA or cDNA in microarrays. Several groups are working on alternative "extra" base pairs to extend the genetic code, such as isoguanine and isocytosine or the fluorescent 2-amino-6-(2-thienyl)purine ...
A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. This succession is denoted by a series of a set of five different letters that indicate the order of the nucleotides. By convention, sequences are usually presented from the 5' end to the 3' end.
To determine the sequence, four types of reversible terminator bases (RT-bases) are added and non-incorporated nucleotides are washed away. A camera takes images of the fluorescently labeled nucleotides. Then the dye, along with the terminal 3' blocker, is chemically removed from the DNA, allowing for the next cycle to begin.
A codon table can be used to translate a genetic code into a sequence of amino acids. [1] [2] The standard genetic code is traditionally represented as an RNA codon table, because when proteins are made in a cell by ribosomes, it is messenger RNA (mRNA) that directs protein synthesis.
The animations have a non-descript musical background and are purposely not narrated to maximize use. These animations may be played alone or simultaneously with the spoken definition of the term. All cellular organelles have animation sequences, as do many key terms such as gene, cell, ACGT, and chromosome. Spoken Explanations of Glossary Terms:
The nucleic acid notation currently in use was first formalized by the International Union of Pure and Applied Chemistry (IUPAC) in 1970. [1] This universally accepted notation uses the Roman characters G, C, A, and T, to represent the four nucleotides commonly found in deoxyribonucleic acids (DNA).
(a) Three hydrogen bonding edges of the four nucleotides (Guanine), showing nomenclature of each edge and (b) Cis and Trans orientations of the sugar moieties of the two nucleotide residues glycosidic bonds of a base pair with respect to hydrogen bonding direction. The arrows in (b) indicate glycosidic bonds as vectors.
For example, MaeII is the prototype enzyme for the sequence "ACGT", with the cleavage site A↓CGT. One of its neoschizomers, Tsp49I, also recognizes the sequence "ACGT", but cleaves at ACGT↓. [2] Another example is SmaI (CCC↓GGG), which is a neoschizomer of XmaI (C↓CCGGG). [3]