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2. 3' mRNA-seq - Wikipedia

   en.wikipedia.org/wiki/3'_mRNA-seq

   Some 3' mRNA-seq technologies, like Bulk RNA Barcoding and Sequencing (BRB-seq) commercialized by Alithea Genomics further streamline the library preparation process by pooling up to 384 samples very early in the workflow for a cost per sample tantamount to profiling four individual genes using conventional qRT-PCR, in a workflow requiring less ...

3. Library (biology) - Wikipedia

   en.wikipedia.org/wiki/Library_(biology)

   A genomic library is a set of clones that together represents the entire genome of a given organism. The number of clones that constitute a genomic library depends on (1) the size of the genome in question and (2) the insert size tolerated by the particular cloning vector system. For most practical purposes, the tissue source of the genomic DNA ...

4. cDNA library - Wikipedia

   en.wikipedia.org/wiki/CDNA_library

   A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism.

5. RNA-Seq - Wikipedia

   en.wikipedia.org/wiki/RNA-Seq

   When sequencing RNA other than mRNA, the library preparation is modified. The cellular RNA is selected based on the desired size range. For small RNA targets, such as miRNA, the RNA is isolated through size selection. This can be performed with a size exclusion gel, through size selection magnetic beads, or with a commercially developed kit.

6. Single-cell sequencing - Wikipedia

   en.wikipedia.org/wiki/Single-cell_sequencing

   To select mRNA, the RT is performed with a single-stranded sequence of deoxythymine (oligo dT) primer which bind specifically the poly(A) tail of mRNA molecules. Subsequently, the amplified cDNA library is used for sequencing. [64] So, the first step of the method is the single cell encapsulation and library preparation.

7. mRNA display - Wikipedia

   en.wikipedia.org/wiki/MRNA_display

   The T7 promoter region allows large-scale in vitro T7 transcription to transcribe the DNA library into an mRNA library, which provides templates for the in vitro translation reaction later. The ribosomal binding site in the 5’-untranslated region (5’ UTR) is designed according to the in vitro translation system to be used.