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2. 3' mRNA-seq - Wikipedia

   en.wikipedia.org/wiki/3'_mRNA-seq

   The sample throughput for 3' mRNA-seq library preparation differs per method but can allow up to 384 samples to be processed in plates, with options for automation. [2] [12] For methods where samples are pooled early in the workflow, consumable use and cost are further reduced. For instance, BRB-seq is up to 25 times cheaper than Illumina ...

3. BRB-seq - Wikipedia

   en.wikipedia.org/wiki/BRB-seq

   Schematic overview of the MERCURIUS BRB-seq workflow where up to 384 samples can be barcoded and multiplexed per kit.. Bulk RNA barcoding and sequencing (BRB-seq) is an ultra-high-throughput bulk 3' mRNA-seq technology that uses early-stage sample barcoding and unique molecular identifiers (UMIs) to allow the pooling of up to 384 samples in one tube early in the sequencing library preparation ...

4. RNA-Seq - Wikipedia

   en.wikipedia.org/wiki/RNA-Seq

   When sequencing RNA other than mRNA, the library preparation is modified. The cellular RNA is selected based on the desired size range. For small RNA targets, such as miRNA, the RNA is isolated through size selection. This can be performed with a size exclusion gel, through size selection magnetic beads, or with a commercially developed kit.

5. List of RNA-Seq bioinformatics tools - Wikipedia

   en.wikipedia.org/wiki/List_of_RNA-Seq...

   qRNASeq script The qRNAseq tool can be used to accurately eliminate PCR duplicates from RNA-Seq data if Molecular Indexes™ or other stochastic labels have been used during library prep. SHERA [42] a SHortread Error-Reducing Aligner. XORRO Rapid Paired-End Read Overlapper. DecontaMiner [43] detects contamination in RNA-Seq data.

6. Library (biology) - Wikipedia

   en.wikipedia.org/wiki/Library_(biology)

   A genomic library is a set of clones that together represents the entire genome of a given organism. The number of clones that constitute a genomic library depends on (1) the size of the genome in question and (2) the insert size tolerated by the particular cloning vector system. For most practical purposes, the tissue source of the genomic DNA ...

7. Transcriptomics technologies - Wikipedia

   en.wikipedia.org/wiki/Transcriptomics_technologies

   The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides, and measurement of fluorescent intensity across the array ...