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Typical specimens for cryofixation include small samples of plant or animal tissue, cell suspensions of microorganisms or cultured cells, suspensions of viruses or virus capsids and samples of purified macromolecules, especially proteins. [2] [3] Types of cryo-fixation are freezing-drying, freezing-substitution and freezing-etching.
Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours. Slow programmable freezing was developed during the early 1970s, and eventually resulted in the first human frozen embryo birth in 1984. Since then, machines that ...
The release on December 8, 1998 and subsequent releases through J2SE 5.0 were rebranded retrospectively Java 2 and the version name "J2SE" (Java 2 Platform, Standard Edition) replaced JDK to distinguish the base platform from J2EE (Java 2 Platform, Enterprise Edition) and J2ME (Java 2 Platform, Micro Edition). This was a very significant ...
At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water.
Eclipse OpenJ9 (previously known as IBM J9) is a high performance, scalable, Java virtual machine (JVM) implementation that is fully compliant with the Java Virtual Machine Specification. [ 3 ] OpenJ9 can be built from source, or can be used with pre-built binaries available at the IBM Semeru Runtimes project for a number of platforms including ...
It has since been used in other applications such as cell disruption nanoemulsions, and solid particle size reduction, among others. By using microchannels with fixed geometry, and an intensifier pump, high shear rates are generated that rupture the cells. This method of cell lysis can yield breakage of over 90% of E. coli cells. [9]
This method relies on the mechanism of freeze dehydration to pull water out of the cells and thus prevent ice formation in the cell. [9] Vitrification. By freezing at an ultra-fast rate and using osmotic dehydration, the water that is still present in the cell is unable to form crystals and will be part of a glass-like or vitrified solution. [10]
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