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In Meristem culture, the meristem and a few subtending leaf primordia are placed into a suitable growing media. where they are induced to form new meristem. These meristems are then divided and further grown and multiplied. To produce plantlets the meristems are taken of from their proliferation medium and put on a regeneration medium.
Tissue culture is the growth of tissues or cells in an artificial medium separate from the parent organism. This technique is also called micropropagation. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
The composition of the medium, particularly the plant hormones and the nitrogen source (nitrate versus ammonium salts or amino acids) have profound effects on the morphology of the tissues that grow from the initial explant. For example, an excess of auxin will often result in a proliferation of roots, while an excess of cytokinin may yield shoots.
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is the most popular plant growth medium used in the laboratories worldwide for cultivation of plant cell culture on agar. MS0 was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS ...
Plant propagation is the process by which new plants grow from various sources, including seeds, cuttings, and other plant parts. Plant propagation can refer to both man-made and natural processes. Propagation typically occurs as a step in the overall cycle of plant growth.
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Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones. [1] It is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia. [2] [3] [4] It has utility for research and clinical care. It was created by, and is named after, Raymond Sabouraud in 1892.