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Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
Humana published more than 100 new books and 25 journals per year [when?] with a backlist of approximately 1,500 titles [when?] in areas such as molecular biology, neuroscience, and medicine. [1] The company was founded in 1976 in Clifton, New Jersey, by Thomas L. Lanigan and his wife, Julia Lanigan, both chemists, and published its first book ...
Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining: Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a ...
It is used in molecular biology, forensics, and medicine for the rapid identification and diagnosis of pathogenic organisms. In contrast to similar techniques such as polymerase chain reaction and ligase chain reaction , this method involves RNA transcription (via RNA polymerase ) and DNA synthesis (via reverse transcriptase ) to produce an RNA ...
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to identify RNA binding sites of proteins on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks.
Protein purification is a critical process in molecular biology and biochemistry, aimed at isolating a specific protein from a complex mixture, such as cell lysates or tissue extracts. [9] The goal is to obtain the protein in a pure form that retains its biological activity for further study, including functional assays, structural analysis, or ...
Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
"Fast NJ" remembers the best node and is O(n^2) always; "relax NJ" performs a hill-climbing search and retains the worst-case complexity of O(n^3). Rapid NJ is faster than plain relaxed NJ. [14] FastME is an implementation of the closely related balanced minimum evolution (BME) method (see § Neighbor joining as minimum evolution). It is about ...