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Paper chromatography is an analytical method used to separate coloured ... The mobile phase is a solution that travels up the stationary phase by capillary action ...
Capillary action of water (polar) compared to mercury (non-polar), in each case with respect to a polar surface such as glass (≡Si–OH). Capillary action (sometimes called capillarity, capillary motion, capillary rise, capillary effect, or wicking) is the process of a liquid flowing in a narrow space without the assistance of external forces like gravity.
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
This solvent then moves up the plate via capillary action. [4] As with all chromatography, some compounds are more attracted to the mobile phase, while others are more attracted to the stationary phase. [5] Therefore, different compounds move up the TLC plate at different speeds and become separated. [6]
Buffer transfer by capillary action from a region of high water potential to a region of low water potential (usually filter paper and paper tissues) is then used to move the DNA from the gel onto the membrane; ion exchange interactions bind the DNA to the membrane due to the negative charge of the DNA and positive charge of the membrane.
The equation is derived for capillary flow in a cylindrical tube in the absence of a gravitational field, but is sufficiently accurate in many cases when the capillary force is still significantly greater than the gravitational force. In his paper from 1921 Washburn applies Poiseuille's Law for fluid motion in a
Paper-based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that transport fluid from an inlet through the porous medium to a desired outlet or region of the device, by means of capillary action. [1]
Capillary rise or fall in a tube. Jurin's law, or capillary rise, is the simplest analysis of capillary action—the induced motion of liquids in small channels [1] —and states that the maximum height of a liquid in a capillary tube is inversely proportional to the tube's diameter.