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  2. Beer–Lambert law - Wikipedia

    en.wikipedia.org/wiki/Beer–Lambert_law

    Absorbance within range of 0.2 to 0.5 is ideal to maintain linearity in the Beer–Lambert law. If the radiation is especially intense, nonlinear optical processes can also cause variances. The main reason, however, is that the concentration dependence is in general non-linear and Beer's law is valid only under certain conditions as shown by ...

  3. Absorbance - Wikipedia

    en.wikipedia.org/wiki/Absorbance

    Since the absorbance of a sample is measured as a logarithm, it is directly proportional to the thickness of the sample and to the concentration of the absorbing material in the sample. Some other measures related to absorption, such as transmittance, are measured as a simple ratio so they vary exponentially with the thickness and concentration ...

  4. Colorimeter (chemistry) - Wikipedia

    en.wikipedia.org/wiki/Colorimeter_(chemistry)

    A colorimeter is a device used in colorimetry that measures the absorbance of particular wavelengths of light by a specific solution. [1] [2] It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.

  5. Bradford protein assay - Wikipedia

    en.wikipedia.org/wiki/Bradford_protein_assay

    The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample. [ 6 ] Unlike other protein assays, the Bradford protein assay is less susceptible to interference by various chemical compounds such as sodium, potassium or even carbohydrates like sucrose ...

  6. Ultraviolet–visible spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Ultraviolet–visible...

    The Beer–Lambert law states that the absorbance of a solution is directly proportional to the concentration of the absorbing species in the solution and the path length. [9] Thus, for a fixed path length, UV-Vis spectroscopy can be used to determine the concentration of the absorber in a solution.

  7. Atomic absorption spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Atomic_absorption_spectroscopy

    The difference in intensity is directly proportional to the concentration of the analyte in the sample, following the Beer-Lambert law: * **A = εcl**, where: * A is the absorbance measured. * ε is the molar absorptivity (constant specific to the element and wavelength). * c is the concentration of the analyte.

  8. Absorption spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Absorption_spectroscopy

    Determining the absolute concentration of a compound requires knowledge of the compound's absorption coefficient. The absorption coefficient for some compounds is available from reference sources, and it can also be determined by measuring the spectrum of a calibration standard with a known concentration of the target.

  9. Molar absorption coefficient - Wikipedia

    en.wikipedia.org/wiki/Molar_absorption_coefficient

    c is the molar concentration of those species; ℓ is the path length. Different disciplines have different conventions as to whether absorbance is decadic (10-based) or Napierian (e-based), i.e., defined with respect to the transmission via common logarithm (log 10) or a natural logarithm (ln). The molar absorption coefficient is usually decadic.