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An Orsat gas analyser or Orsat apparatus is a piece of laboratory equipment used to analyse a gas sample (typically fossil fuel flue gas) for its oxygen, carbon monoxide and carbon dioxide content. Although largely replaced by instrumental techniques, the Orsat remains a reliable method of measurement and is relatively simple to use. [1]
The earliest incubators were invented thousands of years ago in ancient Egypt and China, where they were used to keep chicken eggs warm. [1] Use of incubators revolutionized food production, as it allowed chicks to hatch from eggs without requiring that a hen sit on them, thus freeing the hens to lay more eggs in a shorter period of time.
[1] This table displays the steps involved in using a GasPak to create a completely anaerobic environment for incubating an inoculated agar plate. After inoculating the agar plate(s) with bacteria under aseptic conditions, the agar plates are placed in an anaerobic jar that contains components, like the catalyst chamber, that will help facilitate the reaction to eliminate free oxygen and ...
For successful use in a Kipp's apparatus, the solid material has to be available in lumps large enough to stay on the retention plate without falling through its holes. Hydrogen from iron flakes or zinc and hydrochloric acid or diluted sulfuric acid respectively. Carbon dioxide from pieces of marble (calcium carbonate) and hydrochloric acid
Additionally, using Durham tubes to provide evidence of fermentation may not be able to detect slow- or weakly-fermenting organisms when the resultant carbon dioxide diffuses back into the solution as quickly as it is formed, [4] so a negative test using Durham tubes does not indicate decisive physiological significance.
A vortex shaker in use. Anyone employing an incubator shaker (thermal shaker) to grow yeast or bacteria in the laboratory needs to beware that under the usual conditions encountered in the lab, the rate at which oxygen diffuses from the gaseous phase into the shaken liquid phase is too slow to keep up with the rate at which the oxygen is ...