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Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.
Stainless steel and glass are the usual materials for packed columns and quartz or fused silica for capillary columns. Gas chromatography is based on a partition equilibrium of analyte between a solid or viscous liquid stationary phase (often a liquid silicone-based material) and a mobile gas (most often helium).
The components of a capillary electrochromatograph are a sample vial, source and destination vials, a packed capillary, electrodes, a high voltage power supply, a detector, and a data output and handling device. The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution.
The same equation applies in chromatography processes as for the packed bed processes, namely: = In packed column chromatography, the HETP may also be calculated with the Van Deemter equation. In capillary column chromatography HETP is given by the Golay equation.
The technique of on-column injection, often used with packed columns, is usually not possible with capillary columns. In the injection system in the capillary gas chromatograph the amount injected should not overload the column and the width of the injected plug should be small compared to the spreading due to the chromatographic process.
Narrow-bore columns (1–2 mm) are used for applications when more sensitivity is desired either with special UV-vis detectors, fluorescence detection or with other detection methods like liquid chromatography-mass spectrometry. Capillary columns (under 0.3 mm) are used almost exclusively with alternative detection means such as mass spectrometry.
English: Veins and Capillaries Tissue fluid is the liquid component of blood that flows past the capillary wall to bathe tissue cells. Water, sugars, salts, fatty acids, amino acids, coenzymes, and hormones, as well as waste products from the cells, make up the majority of it.
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