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Functional ultrasound imaging (fUS) is a medical ultrasound imaging technique for detecting or measuring changes in neural activities or metabolism, such as brain activity loci, typically through measuring hemodynamic (blood flow) changes.
Ultrasound Localization Microscopy (ULM) is an advanced ultrasound imaging technique. By localizing microbubbles, ULM overcomes the physical limit of diffraction, achieving sub-wavelength level resolution and qualifying as a super-resolution technique. [1] [2] ULM is primarily utilized in vascular imaging.
English: The main applications and features of functional ultrasound (fUS) imaging. fUS imaging provides (i) a compatibility with a wide range of animal models for preclinical studies, (ii) the ability to image awake and freely moving animals, (iii) possibility to combine with super-resolution ultrasound localization microscopy, (iv) possible extension to 3D imaging, (v) functional ...
English: Main brain functional imaging techniques on a three-axis chart (temporal resolution, spatial resolution, portability). Techniques were separated between local and whole-brain imaging. Functional ultrasound fills a gap between whole brain imaging and microscopy, as well as between fMRI and Optics. [1]
Functional ultrasound imaging (fUS) is a medical ultrasound imaging technique of detecting or measuring changes in neural activities or metabolism, for example, the loci of brain activity, typically through measuring blood flow or hemodynamic changes. Functional ultrasound relies on Ultrasensitive Doppler and ultrafast ultrasound imaging which ...
In comparison to other super-resolution microscopy techniques such as STORM or PALM that rely on single-molecule localization and hence only allow one active molecule per diffraction-limited area (DLA) and timepoint, [1] [2] SOFI does not necessitate a controlled photoswitching and/ or photoactivation as well as long imaging times.
The two dimensional image of a point source observed under a microscope is an extended spot, corresponding to the Airy disk (a section of the point spread function) of the imaging system. The ability to identify as two individual entities two closely spaced fluorophores is limited by the diffraction of light.
Current research in molecular imaging involves cellular/molecular biology, chemistry, and medical physics, and is focused on: 1) developing imaging methods to detect previously undetectable types of molecules, 2) expanding the number and types of contrast agents available, and 3) developing functional contrast agents that provide information ...