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(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid ...
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [ 1 ] as it reduces the risk of splashing, foam or bubble formation.
Generally, a pipette has optimal accuracy from 35-100% of its nominal volume, and should not be used below 10% of that volume. [5] For example, using a 1,000 μl pipette for 50 μl of liquid is not ideal, and using a 100 μl pipette will give better results. [6] Other factors like tip angle and immersion depth may also impact accuracy ...
Pasteur pipettes are commonly used in the medical lab because of its essential accuracy. The design of the Pasteur pipette allows for high effective performance in the medical lab. It produces a constant volume of drop. This reduces the concern of liquid remaining in the pipette. [15]