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Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg 2+.
Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio.
The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...
The new protocol used emulsion to compartmentalize amplification reactions in a single tube. This change made it possible for scientists to scale the method to thousands of reactions in a single run. [107] [108] [109]
Technical Report 069 (TR-069) is a document by the Broadband Forum that specifies the CPE WAN Management Protocol (CWMP). CWMP is a SOAP -based protocol for communication between an internet service provider auto configuration server (ACS) and customer-premises equipment (CPE).
New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. [3]
According to The New York Times, the Army has started to "wikify" certain field manuals, allowing any authorized user to update the manuals. [4] This process, specifically using the MediaWiki arm of the military's professional networking application, milSuite, was recognized by the White House as an Open Government Initiative in 2010.