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Ultrasound Localization Microscopy (ULM) is an advanced ultrasound imaging technique. By localizing microbubbles, ULM overcomes the physical limit of diffraction, achieving sub-wavelength level resolution and qualifying as a super-resolution technique. [1] [2] ULM is primarily utilized in vascular imaging.
Ultrasound Doppler imaging can be used to obtain basic functional measurements of brain activity using blood flow. In functional transcranial Doppler sonography, a low frequency (1-3 MHz) transducer is used through the temporal bone window with a conventional pulse Doppler mode to estimate blood flow at a single focal location.
English: The main applications and features of functional ultrasound (fUS) imaging. fUS imaging provides (i) a compatibility with a wide range of animal models for preclinical studies, (ii) the ability to image awake and freely moving animals, (iii) possibility to combine with super-resolution ultrasound localization microscopy, (iv) possible extension to 3D imaging, (v) functional ...
In comparison to other super-resolution microscopy techniques such as STORM or PALM that rely on single-molecule localization and hence only allow one active molecule per diffraction-limited area (DLA) and timepoint, [1] [2] SOFI does not necessitate a controlled photoswitching and/ or photoactivation as well as long imaging times.
Ultrasound image showing the liver, gallbladder and common bile duct. Medical ultrasound uses high frequency broadband sound waves in the megahertz range that are reflected by tissue to varying degrees to produce (up to 3D) images. This is commonly associated with imaging the fetus in pregnant women. Uses of ultrasound are much broader, however.
This technique also contributes to improving the treatment of these disorders by optimizing the pre-clinical and clinical tests of new medication. They are also expected to have a major economic impact due to earlier and more precise diagnosis. Molecular and Functional Imaging has taken on a new direction since the description of the human genome.
Photo-activated localization microscopy (PALM or FPALM) [1] [2] and stochastic optical reconstruction microscopy (STORM) [3] are widefield (as opposed to point scanning techniques such as laser scanning confocal microscopy) fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit.
Multi-spectral. MSOT collects images at multiple wavelengths and resolves the spectral signatures in each voxel imaged, making it a multi-spectral method. Typically, MSOT is used to generate three images: one anatomical image at a single wavelength, one functional image resolving oxy- and deoxy-hemoglobin concentrations, and a third image resolving additional target photoabsorber(s).