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A phytase (myo-inositol hexakisphosphate phosphohydrolase) is any type of phosphatase enzyme that catalyzes the hydrolysis of phytic acid (myo-inositol hexakisphosphate) – an indigestible, organic form of phosphorus that is found in many plant tissues, especially in grains and oil seeds – and releases a usable form of inorganic phosphorus. [1]
The enzyme 4-phytase (EC 3.1.3.26) catalyzes the following reaction: [1] myo -inositol hexakisphosphate + H 2 O ⇌ {\displaystyle \rightleftharpoons } 1 D - myo -inositol 1,2,3,5,6-pentakisphosphate + phosphate
Of the hundreds of phytase enzymes that have been characterized in the literature, only two have been characterized as 5-phytases. [2] [needs update] A histidine acid phosphatases purified from lily pollen [1] and a protein tyrosine phosphatase-like phytase from Selenomonas ruminantium subsp. lactilytica [2] were both found to have specificity for the 5-phosphate position of myo-inositol ...
The hexavalent phytate anion. Phytic acid was discovered in 1903. [2]Generally, phosphorus and inositol in phytate form are not bioavailable to non-ruminant animals because these animals lack the enzyme phytase required to hydrolyze the inositol-phosphate linkages.
As their alternative name alkaline phytase suggests, BPPs work best in basic (or neutral) environment. Their pH optima is 6–9, [2] which is unique among the ...
PRINCIPLE Determination of phytase activity is based on the colorimetrical quantification at 700nm of free phosphorus released by the hydrolysis of phytate using ammonium molybdate as color reagent. 1 U is the amount of enzyme that liberates 1 umol inorganic orthophosphate per minute under test conditions (pH5.0; temperature 37℃; and ...
The enzyme phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase (EC 3.1.3.67) catalyzes the chemical reaction. 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H 2 O = 1-phosphatidyl-1D-myoinositol 4,5-bisphosphate + phosphate
In biochemistry, an esterase is a class of enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis (and as such, it is a type of hydrolase).