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As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...
Oil immersion objective lenses look superficially identical to non-oil immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...
All enlargers consist of a light source, normally an incandescent light bulb shining though a condenser or translucent screen to provide even illumination, a holder for the negative or transparency, and a specialized lens for projection, though some, such as the Rapid Rectilinear or Aplanat [citation needed] could be used in both camera and enlarger.
Köhler illumination requires several optical components to function: Collector lens and/or field lens; Field diaphragm; Condenser diaphragm; Condenser lens; Schematics of Köhler illumination. Top: Illumination beam path, conjugated planes marked with the light green bar. Bottom: Imaging beam path, conjugated planes marked with the light blue bar.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
The condenser is a lens designed to focus light from the illumination source onto the sample. ... is a light optical process of the so-called point spread function ...
These lenses are often color coded for easier use. The least powerful lens is called the scanning objective lens, and is typically a 4× objective. The second lens is referred to as the small objective lens and is typically a 10× lens. The most powerful lens out of the three is referred to as the large objective lens and is typically 40–100×.
In microscopy, NA is important because it indicates the resolving power of a lens. The size of the finest detail that can be resolved (the resolution) is proportional to λ / 2NA , where λ is the wavelength of the light. A lens with a larger numerical aperture will be able to visualize finer details than a lens with a smaller numerical ...