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A condenser (right) and its respective diaphragm (left) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes.
Due to Snell's law, the numerical aperture remains the same: NA = n 1 sin θ 1 = n 2 sin θ 2. In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light.
The sampling aperture can be a literal optical aperture, that is, a small opening in space, or it can be a time-domain aperture for sampling a signal waveform. For example, film grain is quantified as graininess via a measurement of film density fluctuations as seen through a 0.048 mm sampling aperture.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
The ability of a lens to resolve detail is usually determined by the quality of the lens, but is ultimately limited by diffraction.Light coming from a point source in the object diffracts through the lens aperture such that it forms a diffraction pattern in the image, which has a central spot and surrounding bright rings, separated by dark nulls; this pattern is known as an Airy pattern, and ...
State of the art objectives can have a numerical aperture of up to 0.95. Because sin α 0 is always less than or equal to unity (the number "1"), the numerical aperture can never be greater than unity for an objective lens in air. If the space between the objective lens and the specimen is filled with oil however, the numerical aperture can ...
The aperture affects not only the amount of light that passes through the lens, but also the depth of field of the resulting image: a larger aperture (a smaller f-number, e.g. f/2.0) will have a shallow depth of field, while a smaller aperture (a larger f-number, e.g. f/11) will have a greater depth of field. [3]