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Unidirectional or tandem overlap: the 3' end of one gene overlaps with the 5' end of another gene on the same strand. This arrangement can be symbolized with the notation → → where arrows indicate the reading frame from start to end. Convergent or end-on overlap: the 3' ends of the two genes overlap on opposite strands. This can be written ...
A contig (from contiguous) is a set of overlapping DNA segments that together represent a consensus region of DNA. [1] In bottom-up sequencing projects, a contig refers to overlapping sequence data (); [2] in top-down sequencing projects, contig refers to the overlapping clones that form a physical map of the genome that is used to guide sequencing and assembly. [3]
Only the duplex without overlap at the 5' end will allow extension by DNA polymerase in 3' to 5' direction. Following the extension of the OE-PCR reaction, the PCR mix or the eluted fragments of appropriate size are subject to normal PCR, using the outermost primers used in the initial, mutagenic PCR reactions.
This is the most common form of end lap and is used most in framing. For a half lap in which the members are parallel, the joint may be known as a half lap splice. This is a splice joint and is an alternative to scarfing when joining shorter members end to end. Both members in an end lap have one shoulder and one cheek each. Use for:
A single-stranded non-circular DNA molecule has two non-identical ends, the 3' end and the 5' end (usually pronounced "three prime end" and "five prime end"). The numbers refer to the numbering of carbon atoms in the deoxyribose, which is a sugar forming an important part of the backbone of the DNA molecule.
Broader application benefited from pairwise end sequencing, known colloquially as double-barrel shotgun sequencing.As sequencing projects began to take on longer and more complicated DNA sequences, multiple groups began to realize that useful information could be obtained by sequencing both ends of a fragment of DNA.
The end of a TCU is a point where the turn may end and a new speaker may begin, known as a transition relevance place or TRP. The turn allocation component comprises techniques that select the next speaker. There are two types of techniques: those where the current speaker selects the next speaker, and those where the next speaker selects themself.
In a simple case, the intervals do not overlap and they can be inserted into a simple binary search tree and queried in () time. However, with arbitrarily overlapping intervals, there is no way to compare two intervals for insertion into the tree since orderings sorted by the beginning points or the ending points may be different.