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  2. ATP test - Wikipedia

    en.wikipedia.org/wiki/ATP_test

    ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally-occurring firefly enzyme luciferase using a luminometer.

  3. Pyrosequencing - Wikipedia

    en.wikipedia.org/wiki/Pyrosequencing

    A mixture of three enzymes (DNA polymerase, ATP sulfurylase and firefly luciferase) and a nucleotide are added to single stranded DNA to be sequenced and the incorporation of nucleotide is followed by measuring the light emitted. The intensity of the light determines if 0, 1 or more nucleotides have been incorporated, thus showing how many ...

  4. Firefly luciferase - Wikipedia

    en.wikipedia.org/wiki/Firefly_luciferase

    The protein structure of firefly luciferase consists of 550 amino acids in two compact domains: the N-terminal domain and the C-terminal domain. The N-terminal domain is composed of two β-sheets in an αβαβα structure and a β barrel.

  5. TNP-ATP - Wikipedia

    en.wikipedia.org/wiki/TNP-ATP

    TNP-ATP is a fluorescent molecule that is able to determine whether a protein binds to ATP, and the constants associated with that binding. It is primarily used in fluorescence spectroscopy , but is also very useful as an acceptor molecule in FRET , and as a fluorescent probe in fluorescence microscopy and X-ray crystallography .

  6. Uncoupler - Wikipedia

    en.wikipedia.org/wiki/Uncoupler

    An uncoupler or uncoupling agent is a molecule that disrupts oxidative phosphorylation in prokaryotes and mitochondria or photophosphorylation in chloroplasts and cyanobacteria by dissociating the reactions of ATP synthesis from the electron transport chain.

  7. Luciferase - Wikipedia

    en.wikipedia.org/wiki/Luciferase

    Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein.The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. [1]

  8. Automatic test pattern generation - Wikipedia

    en.wikipedia.org/wiki/Automatic_test_pattern...

    ATPG (acronym for both automatic test pattern generation and automatic test pattern generator) is an electronic design automation method or technology used to find an input (or test) sequence that, when applied to a digital circuit, enables automatic test equipment to distinguish between the correct circuit behavior and the faulty circuit behavior caused by defects.

  9. Light meter - Wikipedia

    en.wikipedia.org/wiki/Light_meter

    Use of a light meter for portrait cinematography in a Turkish music video set. A light meter (or illuminometer) is a device used to measure the amount of light.In photography, an exposure meter is a light meter coupled to either a digital or analog calculator which displays the correct shutter speed and f-number for optimum exposure, given a certain lighting situation and film speed.