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  2. Bergey's Manual of Systematic Bacteriology - Wikipedia

    en.wikipedia.org/wiki/Bergey's_Manual_of...

    Bergey's Manual Trust was established in 1936 to sustain the publication of Bergey's Manual of Determinative Bacteriology and supplementary reference works. The Trust also recognizes individuals who have made outstanding contributions to bacterial taxonomy by presentation of the Bergey Award and Bergey Medal, jointly supported by funds from the Trust and from Springer, the publishers of the ...

  3. Ziehl–Neelsen stain - Wikipedia

    en.wikipedia.org/wiki/Ziehl–Neelsen_stain

    The acidity of the dyes causes them to bind more strongly to the cell walls of the bacteria than to other cells or tissues. This results in the selective staining of only those cells that have a high density of cell wall material, such as acid-fast bacteria. [27] The Ziehl-Neelsen stain is a two step staining process. In the first step, the ...

  4. Flowchart - Wikipedia

    en.wikipedia.org/wiki/Flowchart

    A flowchart is a type of diagram that represents a workflow or process. A flowchart can also be defined as a diagrammatic representation of an algorithm, a step-by-step approach to solving a task. The flowchart shows the steps as boxes of various kinds, and their order by connecting the boxes with arrows.

  5. Alkaline lysis - Wikipedia

    en.wikipedia.org/wiki/Alkaline_lysis

    Alkaline lysis is often an initial step in molecular processes. A proper completion of alkaline lysis yields a pure bacterial plasmid. A plasmid is a circular DNA molecule found naturally in bacteria that replicates independently from chromosomal DNA. Plasmids can also less commonly be found in the other two domains: Archaea and Eukarya.

  6. Entner–Doudoroff pathway - Wikipedia

    en.wikipedia.org/wiki/Entner–Doudoroff_pathway

    While anaerobic bacteria must rely on the glycolysis pathway to create a greater percentage of their required ATP thus its 2 ATP production is more favored over the ED pathway's 1 ATP production. [5] Examples of bacteria using the pathway are: Pseudomonas, [8] a genus of Gram-negative bacteria; Azotobacter, [9] a genus of Gram-negative bacteria

  7. Phage display - Wikipedia

    en.wikipedia.org/wiki/Phage_display

    Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated.

  8. Miles and Misra method - Wikipedia

    en.wikipedia.org/wiki/Miles_and_Misra_method

    The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...

  9. Colonial morphology - Wikipedia

    en.wikipedia.org/wiki/Colonial_morphology

    Examining colonial morphology is the first step in the identification of an unknown microbe. The systematic assessment of the colonies' appearance, focusing on aspects like size, shape, colour, opacity, and consistency, provides clues to the identity of the organism, allowing microbiologists to select appropriate tests to provide a definitive ...