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  2. Orbital hybridisation - Wikipedia

    en.wikipedia.org/wiki/Orbital_hybridisation

    For this molecule, carbon sp 2 hybridises, because one π (pi) bond is required for the double bond between the carbons and only three σ bonds are formed per carbon atom. In sp 2 hybridisation the 2s orbital is mixed with only two of the three available 2p orbitals, usually denoted 2p x and 2p y. The third 2p orbital (2p z) remains unhybridised.

  3. Gene flow - Wikipedia

    en.wikipedia.org/wiki/Gene_flow

    Species can live in the same environment, yet show very limited gene flow due to reproductive barriers, fragmentation, specialist pollinators, or limited hybridization or hybridization yielding unfit hybrids. A cryptic species is a species that humans cannot tell is different without the use of genetics.

  4. Nucleic acid hybridization - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_hybridization

    Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).

  5. Valence bond theory - Wikipedia

    en.wikipedia.org/wiki/Valence_bond_theory

    Since the nature of the overlapping orbitals are different in H 2 and F 2 molecules, the bond strength and bond lengths differ between H 2 and F 2 molecules. In methane (CH 4), the carbon atom undergoes sp 3 hybridization, allowing it to form four equivalent sigma bonds with hydrogen atoms, resulting in a tetrahedral geometry. Hybridization ...

  6. Isovalent hybridization - Wikipedia

    en.wikipedia.org/wiki/Isovalent_hybridization

    In chemistry, isovalent or second order hybridization is an extension of orbital hybridization, the mixing of atomic orbitals into hybrid orbitals which can form chemical bonds, to include fractional numbers of atomic orbitals of each type (s, p, d). It allows for a quantitative depiction of bond formation when the molecular geometry deviates ...

  7. Bent's rule - Wikipedia

    en.wikipedia.org/wiki/Bent's_rule

    [5] [22] For instance, a modification of this analysis is still viable, even if the lone pairs of H 2 O are considered to be inequivalent by virtue of their symmetry (i.e., only s, and in-plane p x and p y oxygen AOs are hybridized to form the two O-H bonding orbitals σ O-H and lone pair n O (σ), while p z becomes an inequivalent pure p ...

  8. Genomic library - Wikipedia

    en.wikipedia.org/wiki/Genomic_library

    Colony Blot Hybridization. In order to isolate clones that contain regions of interest from a library, the library must first be screened. One method of screening is hybridization. Each transformed host cell of a library will contain only one vector with one insert of DNA. The whole library can be plated onto a filter over media.

  9. Hybridization assay - Wikipedia

    en.wikipedia.org/wiki/Hybridization_assay

    The dual ligation hybridization assay (DLA) [6] extends the specificity of the hybridization-ligation assay to a specific method for the parent compound. Despite hybridization-ligation assay's robustness, sensitivity and added specificity for the 3'-end of the oligonculeotide analyte, the hybridization-ligation assay is not specific for the 5 ...