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Infobox references. Ethylenediaminetetraacetic acid (EDTA), also called EDTA acid, is an aminopolycarboxylic acid with the formula [CH 2 N (CH 2 CO 2 H) 2] 2. This white, slightly water-soluble solid is widely used to bind to iron (Fe 2+ /Fe 3+) and calcium ions (Ca 2+), forming water-soluble complexes even at neutral pH.
Vacutainer. A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing.
The silica particles are desiccants, which adsorb and hold water vapor. [3] This is used in the tubes so the blood adheres to the surface of the tiny silica particles and begins to clot. After the blood sample is centrifuged, the clear serum should be removed for testing. [4] [5]
In addition, test tubes used for laboratory blood tests will have chemicals added to stop blood clotting. Besides heparin, most of these chemicals bind calcium ions, preventing the coagulation proteins from using them. Ethylenediaminetetraacetic acid (EDTA) strongly and irreversibly chelates (binds) calcium ions, preventing blood from clotting.
In medicine, venipuncture or venepuncture is the process of obtaining intravenous access for the purpose of venous blood sampling (also called phlebotomy) or intravenous therapy. In healthcare, this procedure is performed by medical laboratory scientists, medical practitioners, some EMTs, paramedics, phlebotomists, dialysis technicians, and ...
Test tubes are convenient containers for heating small amounts of liquids or solids with a Bunsen burner or alcohol burner. The tube is usually held by its neck with a clamp or tongs. By tilting the tube, the bottom can be heated to hundreds of degrees in the flame, while the neck remains relatively cool, possibly allowing vapours to condense ...
Blood is composed of blood cells suspended in blood plasma. Plasma, which constitutes 55% of blood fluid, is mostly water (92% by volume), [2] and contains proteins, glucose, mineral ions, and hormones. The blood cells are mainly red blood cells (erythrocytes), white blood cells (leukocytes), and (in mammals) platelets (thrombocytes). [3]
Buffy coat. Layer of thrombocytes and leukocytes ("buffy coat") between erythrocytes and blood plasma after gentle centrifugation of whole blood with sodium ethylenediaminetetraacetate anticoagulant. The buffy coat is the fraction of an anticoagulated blood sample that contains most of the leukocytes and thrombocytes following centrifugation.