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The enzyme alkaline phosphatase (ALP, alkaline phenyl phosphatase, also abbreviated PhoA) is a phosphatase with the physiological role of dephosphorylating compounds. The enzyme is found across a multitude of organisms, prokaryotes and eukaryotes alike, with the same general function, but in different structural forms suitable to the environment they function in. Alkaline phosphatase is found ...
In the initially published VCC experiments [1] only the internal 60 wells of the plate were used, since evaporation changed the volume of the edge wells during the 12-hour incubation. However, all 96 wells of the 96-well plate can be used for the experiment as long as the edge of the plate is wrapped with a piece of Parafilm M six squares long ...
Elevated alkaline phosphatase occurs when levels of alkaline phosphatase (ALP) exceed the reference range. This group of enzymes has a low substrate specificity and catalyzes the hydrolysis of phosphate esters in a basic environment. The major function of alkaline phosphatase is transporting chemicals across cell membranes. [1]
In microbiology, a colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions.
Specific activity gives a measurement of enzyme purity in the mixture. It is the micro moles of product formed by an enzyme in a given amount of time (minutes) under given conditions per milligram of total proteins. Specific activity is equal to the rate of reaction multiplied by the volume of reaction divided by the mass of total protein.
However, when experiments have been carried out in-vivo, phosphatase enzymes have been shown to be incredibly specific. [3] In some cases, a protein phosphatase (i.e. one defined by its recognition of protein substrates) can catalyze the dephosphorylation of nonprotein substrates. [ 4 ]
The cell concentration needs to be known for many experiments in molecular biology, in order to adjust accordingly the amount of reagents and chemicals that are to be applied in the experiment. Studies that examine the growth rate of microorganisms (in other words, how fast they divide to create new cells) require cell counting.
248 11648 Ensembl ENSG00000163295 ENSMUSG00000036500 UniProt P09923 P24822 RefSeq (mRNA) NM_001631 NM_007432 RefSeq (protein) NP_001622 n/a Location (UCSC) Chr 2: 232.46 – 232.46 Mb Chr 1: 87.05 – 87.06 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase that in humans is encoded by the ALPI gene ...