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A volumetric pipette, bulb pipette, or belly pipette [1] allows extremely accurate measurement (to four significant figures) of the volume of a solution. [2] It is calibrated to deliver accurately a fixed volume of liquid. These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a ...
A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision , from single piece glass pipettes to more complex adjustable or ...
A graduated pipette is a pipette with its volume, in increments, marked along the tube. It is used to accurately measure and transfer a volume of liquid from one container to another. [1] It is made from plastic or glass tubes and has a tapered tip. Along the body of the tube are graduation markings indicating volume from the tip to that point.
The fixed volume pipette cannot be changed. As there are less moving parts, the mechanism is less complex, resulting in more accurate volume measurement. In 1972, several people of the University of Wisconsin–Madison (mainly Warren Gilson and Henry Lardy) enhanced the fixed-volume pipette, developing the pipette with a variable volume. [4]
Dip the tip into the solution to a certain depth according to the volume set. Afterwards slowly release the pipette knob till the starting position (#2). Withdraw the tip from the liquid. Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid.
Positive displacement pipettes are a type of pipette that operates via piston-driven displacement. [1] [2] Unlike an air displacement pipette, which dispenses liquid using an air cushion in the pipette tip, the piston in a positive displacement pipette makes direct contact with the sample, allowing the aspiration force to remain constant.