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  2. CRISPR activation - Wikipedia

    en.wikipedia.org/wiki/CRISPR_activation

    CRISPR activation (CRISPRa) is a gene regulation technique that ... This method allows for precise control of gene expression, making it a valuable tool for studying ...

  3. CRISPR/Cas tools - Wikipedia

    en.wikipedia.org/wiki/CRISPR/Cas_Tools

    For a given candidate gRNA, these tools report its list of potential off-targets in the genome thereby allowing the designer to evaluate its suitability prior to embarking on any experiments. Scientists have also begun exploring the mechanics of the CRISPR/Cas system and what governs how good, or active, a gRNA is at directing the Cas nuclease ...

  4. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    Conversely, CRISPR-mediated activation (CRISPRa) promotes gene transcription. [242] Cas9 is an effective way of targeting and silencing specific genes at the DNA level. [243] In bacteria, the presence of Cas9 alone is enough to block transcription. For mammalian applications, a section of protein is added.

  5. Scientists design the next-gen CRISPR for gene editing - AOL

    www.aol.com/news/2016-12-13-next-gen-crispr.html

    The current version of CRISPR uses an enzyme known as Cas9 to cut DNA segments, such as the specific parts that cause genetic disorders. Western's scientists created TevCas9 by combining Cas9 with ...

  6. CRISPR - Wikipedia

    en.wikipedia.org/wiki/CRISPR

    Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within living organisms.

  7. Genome-wide CRISPR-Cas9 knockout screens - Wikipedia

    en.wikipedia.org/wiki/Genome-wide_CRISPR-Cas9...

    The approach utilises the CRISPR-Cas9 gene editing system, coupled with libraries of single guide RNAs (sgRNAs), which are designed to target every gene in the genome. Over recent years, the genome-wide CRISPR screen has emerged as a powerful tool for performing large-scale loss-of-function screens, with low noise, high knockout efficiency and ...