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In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor R F is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [ 2 ]
Retention distance, or R D, is a concept in thin layer chromatography, designed for quantitative measurement of equal-spreading of the spots on the chromatographic plate and one of the Chromatographic response functions. It is calculated from the following formula:
In gas chromatography, the Kovats retention index (shorter Kovats index, retention index; plural retention indices) is used to convert retention times into system-independent constants. The index is named after the Hungarian-born Swiss chemist Ervin Kováts , who outlined the concept in the 1950s while performing research into the composition ...
Retention factor (kappa prime) measures how long a component of the mixture stuck to the column, measured by the area under the curve of its peak in a chromatogram (since HPLC chromatograms are a function of time). Each chromatogram peak will have its own retention factor (e.g., kappa1 for the retention factor of the first peak). This factor ...
Plotted on the x-axis is the retention time and plotted on the y-axis a signal (for example obtained by a spectrophotometer, mass spectrometer or a variety of other detectors) corresponding to the response created by the analytes exiting the system. In the case of an optimal system the signal is proportional to the concentration of the specific ...
The retention factor (R ƒ) may be defined as the ratio of the distance travelled by the solute to the distance travelled by the solvent.It is used in chromatography to quantify the amount of retardation of a sample in a stationary phase relative to a mobile phase. [2]
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The retardation factor (R f), or retention factor, quantifies the results. It is the distance traveled by a given substance divided by the distance traveled by the mobile phase. [citation needed] Development of a TLC plate. Spots that appear purple separate into red spots and blue spots.