Ads
related to: immunofluorescence powerpoint presentationfreelancer.com has been visited by 10K+ users in the past month
- Post Your Project - Free
Post Your Project & Receive Bids
From Freelancers Within Seconds.
- How Freelancer Works
Post Your Project, Choose a
Freelancer, Get Your Job Done!
- Sign Up Now - Free
Join the World's Largest
Freelancing Platform Today.
- Get The Perfect Design
Start a Contest Today - 100s of
Entries, Only Pay for the Best!
- Post Your Project - Free
Search results
Results From The WOW.Com Content Network
Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens . [ 1 ]
They are also conjugated to a special kind of tag that allows the antibody to be visualized in the lab, i.e.so that it will emit fluorescence or a color. Hence, immunofluorescence refers to the detection of a fluorescent antibody (immuno) and immunoperoxidase refers to the detection of a colored antibody (peroxidase produces a dark brown color).
Immunocytochemistry labels individual proteins within cells, such as TH (green) in the axons of sympathetic autonomic neurons.. Immunocytochemistry (ICC) is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it.
Antibody isotype(s) and location of antibody deposition in immunofluorescence studies using salt-split skin for autoimmune bullous conditions targeting the basement membrane zone of the human integumentary system Condition Antibody isotype(s) deposited Localization of antibody with use of salt-split skin Antiepilegrin cicatricial pemphigoid ...
Immunofluorescence staining pattern of anti-dsDNA antibodies on HEp-20-10 cells (left), Crithidia luciliae (centre) and rat liver (right). Anti-double stranded DNA (Anti-dsDNA) antibodies are a group of anti-nuclear antibodies (ANA) the target antigen of which is double stranded DNA.
Immunofluorescence can also be done within the interior of live cells by reversible cell permeabilization using the detergent Triton X-100. Adjusted carefully to the appropriate concentration for the number of cells, the pretreatment can permit access of molecules between 1 and 150 kilodaltons to the interior of the cell. [ 5 ]
Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. [1] Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody .
Immunoperoxidase is a type of immunostain used in molecular biology, medical research, and clinical diagnostics.In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction.