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Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers . [ 3 ]
21752 Ensembl ENSG00000164362 ENSMUSG00000021611 UniProt O14746 O70372 RefSeq (mRNA) NM_001193376 NM_198253 NM_198254 NM_198255 NM_009354 NM_001362387 NM_001362388 RefSeq (protein) NP_001180305 NP_937983 NP_033380 NP_001349316 NP_001349317 Location (UCSC) Chr 5: 1.25 – 1.3 Mb Chr 13: 73.78 – 73.8 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Telomerase reverse transcriptase ...
Bio-Rad Laboratories, Inc. is an American developer and manufacturer of specialized technological products for the life science research and clinical diagnostics markets. The company was founded in 1952 in Berkeley, California , by husband and wife team David and Alice Schwartz , both graduates of the University of California, Berkeley.
Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).
These methods are based on gel filtration chromatography, [2] also called molecular sieve chromatography, which is a form of size-exclusion chromatography. Desalting and buffer exchange are two of the most common gel filtration chromatography applications, and they can be performed using the same resin.
[10] [11] By adding a second restriction enzyme, replacing the random shearing, and a tight DNA size selection step it is possible to perform low-cost population genotyping. This can be an especially powerful tool for whole-genome scans for selection and population differentiation or population adaptation.
DNA repair and recombination protein RAD54-like is a protein that in humans is encoded by the RAD54L gene. [5] [6]The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA.
In the field of genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover single nucleotide polymorphisms (SNP) in order to perform genotyping studies, such as genome-wide association studies (). [1]