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Yeast need a reliable source of nitrogen in forms that they can assimilate in order to successfully complete fermentation. Yeast assimilable nitrogen or YAN is the combination of free amino nitrogen (FAN), ammonia (NH 3) and ammonium (NH 4 +) that is available for a yeast, e.g. the wine yeast Saccharomyces cerevisiae, to use during fermentation.
Microbial food cultures are live bacteria, yeasts or moulds used in food production. Microbial food cultures carry out the fermentation process in foodstuffs. Used by humans since the Neolithic period (around 10 000 years BC) [1] fermentation helps to preserve perishable foods and to improve their nutritional and organoleptic qualities (in this case, taste, sight, smell, touch).
Apart from spoiling foods, as a direct consequent of growth, Z. bailii can modify the product texture and composition such that it may be more readily colonized by other spoilage microorganisms. For example, by utilizing acetic acid, the yeast can raise the pH of pickles sufficiently to allow the growth of less acid-tolerant bacteria. [5]
The French chemist Louis Pasteur founded zymology, when in 1856 he connected yeast to fermentation. [9] When studying the fermentation of sugar to alcohol by yeast, Pasteur concluded that the fermentation was catalyzed by a vital force, called "ferments", within the yeast cells. The "ferments" were thought to function only within living organisms.
Despite the bactericidal effects of ethanol, acidifying effects of fermentation, and low oxygen conditions of industrial alcohol production, bacteria that undergo lactic acid fermentation can contaminate such facilities because lactic acid has a low pKa of 3.86 to avoid decoupling the pH membrane gradient that supports regulated transport. [7]
Humans have used fermentation in production of food for 13,000 years. [5] Humans and their livestock have microbes in the gut that carry out fermentation, releasing products used by the host for energy. [6] Fermentation is used at an industrial level to produce commodity chemicals, such as ethanol and lactate.
Fermentation of sugars by yeast is the oldest and largest application of this technology. Many types of yeasts are used for making many foods: baker's yeast in bread production, brewer's yeast in beer fermentation, and yeast in wine fermentation and for xylitol production. [57] So-called red rice yeast is actually a mold, Monascus purpureus.
Sch. pombe is a Crabtree-positive yeast, which developed aerobic fermentation independently from Saccharomyces lineage, and detects glucose via the cAMP-signaling pathway. [20] The number of transporter genes vary significantly between yeast species and has continually increased during the evolution of the S. cerevisiae lineage.