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Typical dot blot membrane. Darker dots indicate more protein. A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Instead, the sample is applied directly on ...
A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available. [1]
Protein:protein interaction studies; Colony blot, dot blot, Western blot and ELISA; Screening for positive expression clones; Immunocytochemistry and Immunohistochemistry; Protein localization and targeting studies; Because the Strep-tag is capable of isolating protein complexes, strategies for the study of protein-protein interactions can also ...
Alternatively, if the target is already suspected and is being tested for validation, a western blot protocol can be used to identify protein directly. [18] An example stability of proteins from rates of oxidation (SPROX) workflow. Binding of a drug to a protein often leads to ligand-induced stabilization of the protein.
[1] This technique is considered to be more sensitive than primary immunofluorescence, because multiple secondary antibodies can bind to the same primary antibody. The increased number of fluorophore molecules per antigen increases the amount of emitted light, and thus amplifies the signal. [1]
It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler dot blot assay. It is a common tool used in genetic testing, forensics, and molecular biology research. An ASO is typically an oligonucleotide of 15–21 nucleotide bases in length.
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