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NCO recombinants are thought to occur primarily by the Synthesis Dependent Strand Annealing (SDSA) model, illustrated on the left. Most recombination events appear to be the SDSA type. Genetic recombination (also known as genetic reshuffling ) is the exchange of genetic material between different organisms which leads to production of offspring ...
Recombinant DNA differs from genetic recombination in that the former results from artificial methods while the latter is a normal biological process that results in the remixing of existing DNA sequences in essentially all organisms.
Gene conversion is the process by which one DNA sequence replaces a homologous sequence such that the sequences become identical after the conversion. [1] Gene conversion can be either allelic, meaning that one allele of the same gene replaces another allele, or ectopic, meaning that one paralogous DNA sequence converts another.
These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. [2] Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses. Horizontal gene transfer is the ...
The final result of conjugation, transduction, and/or transformation is the production of genetic recombinants, individuals that carry not only the genes they inherited from their parent cells but also the genes introduced to their genomes by conjugation, transduction, and/or transformation.
A model experiment in genetics using the Cre-lox system: the premature stop sequence present in floxed mice is removed only from cells that express Cre recombinase when the mice are bred together. Initiation of site-specific recombination begins with the binding of recombination proteins to their respective DNA targets.
In genetics, the coefficient of coincidence (c.o.c.) is a measure of interference in the formation of chromosomal crossovers during meiosis. It is generally the case that, if there is a crossover at one spot on a chromosome, this decreases the likelihood of a crossover in a nearby spot. [1] This is called interference.
Construction of a genomic library involves creating many recombinant DNA molecules. An organism's genomic DNA is extracted and then digested with a restriction enzyme.For organisms with very small genomes (~10 kb), the digested fragments can be separated by gel electrophoresis.