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Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
Generally speaking, temperature and light are already going to be standards of control practiced in micropropagation. However, ventilation and CO 2 concentration become a challenge, especially while trying to maintain sterile conditions (even though if there is no sugar in the growing medium, aseptic technique is still an essential practice ...
The propagation of shoots or nodal segments is usually performed in four stages for mass production of plantlets through in vitro vegetative multiplication but organogenesis is a standard method of micropropagation that involves tissue regeneration of adventitious organs or axillary buds directly or indirectly from the explants.
This technique is also called micropropagation. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants.
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English: This is a simple micropropagation technique in tissue culture. Its overall steps consist of obtaining a sample from a parent plant, placing that sample into a prepared culture with nutrients in order to develop callus, shoot multiplication, rooting of the shoots, and transferring plantlets into the soil.
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Lilian Pateña is a Filipino scientist who discovered a breed of calamansi and seedless pomelo and discovered micropropagation which established the banana industry in the Philippines. She is also an inventor of leaf-bud cutting in cassava. She was recognized as one of The Outstanding Women in the Nation's Service (TOWNS) in 1998, Women of ...