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FISH can also be used to detect diseased cells more easily than standard Cytogenetic methods, which require dividing cells and requires labor and time-intensive manual preparation and analysis of the slides by a technologist. FISH, on the other hand, does not require living cells and can be quantified automatically, a computer counts the ...
Using cells in culture; Pre-treating cells in a hypotonic solution, which swells them and spreads the chromosomes; Arresting mitosis in metaphase by a solution of colchicine; Squashing the preparation on the slide forcing the chromosomes into a single plane; Cutting up a photomicrograph and arranging the result into an indisputable karyogram.
Organ culture is the cultivation of either whole organs or parts of organs in vitro. [1] It is a development from tissue culture methods of research, as the use of the actual in vitro organ itself allows for more accurate modelling of the functions of an organ in various states and conditions. [2]
The primary advantage of this technique is that it eliminates the time required in Q-FISH to prepare metaphase spreads of cells of interest, and that flow cytometric analysis is also considerably faster than the methods required to acquire and analyze Q-FISH prepared slides. Flow-FISH thus allows for a higher throughput analysis of telomere ...
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Raceway systems are among the earliest methods used for inland aquaculture. A raceway usually consists of rectangular basins or canals constructed of concrete and equipped with an inlet and outlet. A continuous water flow-through is maintained to provide the required level of water quality, which allows animals to be cultured at higher ...
The pronephros is a functioning kidney of the embryo in bony fish and amphibian larvae, [7] but in mammals it is most often considered rudimentary and not functional. [18] In some lungfish and bony fishes, the pronephros can remain functional in adults, including often simultaneously with the mesonephros. [ 7 ]
In cartilaginous fish, there is also a shorter duct which drains the posterior (metanephric) parts of the kidney, and joins with the mesonephric duct at the bladder or cloaca. Indeed, in many cartilaginous fish, the anterior portion of the kidney may degenerate or cease to function altogether in the adult. [ 35 ]