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Django (/ ˈ dʒ æ ŋ ɡ oʊ / JANG-goh; sometimes stylized as django) [5] is a free and open-source, Python-based web framework that runs on a web server. It follows the model–template–views (MTV) architectural pattern .
A carousel slide projector. The example pictured is a Kodak Carousel model 4400, dating from the mid-1980s. A carousel slide projector is a slide projector that uses a rotary tray to store slides, used to project slide photographs and to create slideshows. It was first patented on May 11, 1965, by David E. Hansen of Fairport, New York.
In the I-E system of E. coli, Cas1 and Cas2 form a complex where a Cas2 dimer bridges two Cas1 dimers. [116] In this complex, Cas2 performs a non-enzymatic scaffolding role, [ 116 ] binding double-stranded fragments of invading DNA, while Cas1 binds the single-stranded flanks of the DNA and catalyses their integration into CRISPR arrays.
Multiple crRNAs and the tracrRNA can be packaged together to form a single-guide RNA (sgRNA). [59] This sgRNA can be included alongside the gene that codes for the Cas9 protein and made into a plasmid in order to be transfected into cells. Many online tools are available to aid in designing effective sgRNA sequences. [60] [61]
Django Unchained (/ ˈ dʒ æ ŋ ɡ oʊ /) is a 2012 American revisionist Western [5] film written and directed by Quentin Tarantino.Produced by Tarantino's A Band Apart and Columbia Pictures, it stars Jamie Foxx, Christoph Waltz, Leonardo DiCaprio, Kerry Washington, and Samuel L. Jackson; Walton Goggins, Dennis Christopher, James Remar, Michael Parks, and Don Johnson also star in supporting ...