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HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Andrew Alpert in his 1990 paper on the subject. [ 2 ] He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity, a conclusion supported by a review and re ...
Chromatography, pronounced / ˌ k r oʊ m ə ˈ t ɒ ɡ r ə f i /, is derived from Greek χρῶμα chroma, which means "color", and γράφειν graphein, which means "to write".The combination of these two terms was directly inherited from the invention of the technique first used to separate biological pigments.
Electrolysis of water is using electricity to split water into oxygen (O 2) and hydrogen (H 2) gas by electrolysis. Hydrogen gas released in this way can be used as hydrogen fuel, but must be kept apart from the oxygen as the mixture would be extremely explosive.
Conversely, hydrophilic drugs (low octanol/water partition coefficients) are found primarily in aqueous regions such as blood serum. [ 4 ] If one of the solvents is a gas and the other a liquid, a gas/liquid partition coefficient can be determined.
After protein folding in aqueous solution, hydrophobic amino acids usually form protected hydrophobic areas while hydrophilic amino acids interact with the molecules of solvation and allow proteins to form hydrogen bonds with the surrounding water molecules. If enough of the protein surface is hydrophilic, the protein can be dissolved in water. [4]
Water (H 2 O) is a polar inorganic compound that is at room temperature a tasteless and odorless liquid, which is nearly colorless apart from an inherent hint of blue.It is by far the most studied chemical compound [20] and is described as the "universal solvent" [21] and the "solvent of life". [22]
The octanol-water partition coefficient, usually expressed as its logarithm (Log P), is a measure of differential solubility of a compound in a hydrophobic solvent and a hydrophilic solvent (water). The logarithm of these two values enables compounds to be ranked in terms of hydrophilicity (or hydrophobicity).
Picture of an SDS-PAGE. The molecular markers (ladder) are in the left lane. Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.