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UV–visible spectroscopy of microscopic samples is done by integrating an optical microscope with UV–visible optics, white light sources, a monochromator, and a sensitive detector such as a charge-coupled device (CCD) or photomultiplier tube (PMT). As only a single optical path is available, these are single beam instruments.
UV can also induce infrared fluorescence and UV fluorescence depending on the subject. For UV induced non-visible fluorescence photography, a camera must be modified in order to capture UV or IR images, and UV or IR capable lenses must be used. Filters are sometimes added to the UV illumination source to narrow the illuminant waveband.
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.
This image was then viewed through a tube with a scale that was transposed upon the spectral image, enabling its direct measurement. With the development of photographic film, the more accurate spectrograph was created. It was based on the same principle as the spectroscope, but it had a camera in place of the viewing tube.
Comparison of images taken with different spectral responses. Digital sensors and photographic films can be made to record non-visible ultraviolet (UV) and infrared (IR) radiation. In each case, they generally require special equipment: converted digital cameras, specific filters, highly transmitting lenses, etc.