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SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa.
Calcium carbonate precipitates as a solid, leaving ammonium sulfate in the solution. (NH 4) 2 CO 3 + CaSO 4 → (NH 4) 2 SO 4 + CaCO 3. Ammonium sulfate occurs naturally as the rare mineral mascagnite in volcanic fumaroles and due to coal fires on some dumps. [14] Ammonium sulfate is a byproduct in the production of methyl methacrylate. [15]
ABC dry chemical is usually a mix of monoammonium phosphate and ammonium sulfate, the former being the active component. The mix between the two agents is usually 40–60%, 60–40%, or 90–10% depending on local standards worldwide. The USGS uses a similar mixture, called Phos Chek G75F. [2]
Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH + 4) and sulfate (SO 2− 4) in aqueous solutions. [1] Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive.
NaOH reacts the ammonium (NH 4 +) to ammonia (NH 3), which boils off the sample solution. Ammonia bubbles through the standard acid solution and reacts back to ammonium salts with the weak or strong acid. [3] Ammonium ion concentration in the acid solution, and thus the amount of nitrogen in the sample, is measured via titration.
Ammonium iron(III) sulfate, NH 4 Fe(SO 4) 2 ·12 H 2 O, or NH 4 [Fe(H 2 O) 6](SO 4) 2 ·6 H 2 O, also known as ferric ammonium sulfate (FAS) or iron alum, is a double salt in the class of alums, which consists of compounds with the general formula AB(SO 4) 2 · 12 H 2 O. [2] It has the appearance of weakly violet, octahedrical crystals.
Sodium dodecyl sulfate (SDS) or sodium lauryl sulfate (SLS), sometimes written sodium laurilsulfate, is an organic compound with the formula CH 3 (CH 2) 11 OSO 3 Na and structure H 3 C−(CH 2) 11 −O−S(=O) 2 −O − Na +. It is an anionic surfactant used in many cleaning and hygiene products. This compound is the sodium salt of the 12 ...
Tetramethylammonium hydroxide (TMAH or TMAOH) is a quaternary ammonium salt with molecular formula N(CH 3) 4 + OH −.It is commonly encountered in form of concentrated solutions in water or methanol.