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India ink staining, also known as negative staining, is a simple yet effective approach for visualizing the exterior structures of bacteria, fungi, and other microbes in microbiology. The dye is used to stain the background in this staining process, while the microbe shows as a clear patch against the dark background.
A. India ink method. In this method, two dyes, crystal violet, and India ink are used. The capsule is seen as a clear halo around the microorganism against the black background. This method is used for demonstrating Cryptococcus. The background will be dark (color of India ink).
The India ink test is a simple yet powerful diagnostic tool used in medical microbiology to detect Cryptococcus, a type of encapsulated yeast that can cause serious infections in humans. This test uses the principle of negative staining to visualize the capsule of Cryptococcus cells, allowing for their identification and differentiation from ...
Negative staining requires an acidic dye such as India Ink or Nigrosin. India Ink or Nigrosin is an acidic stain. This means that the stain readily gives up a hydrogen ion (proton) and the chromophore of the dye becomes negatively charged.
One very simple approach is mixing cells in a preparation of India ink. The large particles of ink will not penetrate the tight layers of the capsule or stain the bacterium. The particles of the ink will however provide a negative background that allows visualization of cells and capsules.
India ink or nigrosin stain. Clean and grease-free slide and coverslips. Test specimen-CSF.
Place a small drop of a negative stain (India Ink, Congo Red, Nigrosin, or Eosin) on the slide. Congo Red is easier to see, but it does not work well with some strains. India Ink generally works, but it has tiny particles that display Brownian motion that must be differentiated from your bacteria.