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The first impedance-based flow cytometry device, using the Coulter principle, was disclosed in U.S. Patent 2,656,508, issued in 1953, to Wallace H. Coulter. Mack Fulwyler was the inventor of the forerunner to today's flow cytometers – particularly the cell sorter. [6] Fulwyler developed this in 1965 with his publication in Science. [7]
A Coulter counter played an important role in the development of the first cell sorter, and was involved in the early development of flow cytometry. Some flow cytometers continue to utilize the Coulter principle to provide information about cell size and count.
This type of hematology analyzer utilizes both Coulter's principle and flow cytometry to determine the granularity, diameter, and inner complexity of the cells. Using hydrodynamic focusing, the cells are sent through an aperture one cell at a time.
[5] [13] This principle is today known as the Coulter principle and was used in the automated blood cell counter released by Coulter Electronics in 1954. The “Coulter counter” was the first commercial flow cytometer. [citation needed] During the 1960s Dittrich, Göhde and Kamentsky improves the design pioneered by Caspersson 30 years earlier.
Hydrodynamic focusing solves this problem by building up the walls of the tunnel from fluid, using the effects of fluid dynamics.A wide (hundreds of micrometers in diameter) tube made of glass or plastic is used, through which a "wall" of fluid called the sheath flow is pumped.
Beckman Coulter, Inc. is a Danaher Corporation company that develops, ... the company acquired the Flow Cytometry Business Group of Dako North America, Inc.
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