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  2. Focal length - Wikipedia

    en.wikipedia.org/wiki/Focal_length

    The focal point F and focal length f of a positive (convex) lens, a negative (concave) lens, a concave mirror, and a convex mirror.. The focal length of an optical system is a measure of how strongly the system converges or diverges light; it is the inverse of the system's optical power.

  3. Microscopic scale - Wikipedia

    en.wikipedia.org/wiki/Microscopic_scale

    The ratio of the focal length of the objective and the eyepiece, when mounted in a standard tube length, gives an approximate magnification of the system. Due to their design, compound microscopes have improved resolving power and contrast in comparison to simple microscopes, [11] and can be used to view the structure, shape and motility of a ...

  4. Objective (optics) - Wikipedia

    en.wikipedia.org/wiki/Objective_(optics)

    Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...

  5. f-number - Wikipedia

    en.wikipedia.org/wiki/F-number

    A 100 mm focal length f /4 lens has an entrance pupil diameter of 25 mm. A 100 mm focal length f /2 lens has an entrance pupil diameter of 50 mm. Since the area is proportional to the square of the pupil diameter, [6] the amount of light admitted by the f /2 lens is four times that of the f /4 lens.

  6. Optical unit - Wikipedia

    en.wikipedia.org/wiki/Optical_unit

    Optical units are dimensionless units of length used in optical microscopy. They are used to express distances in terms of the numerical aperture of the system and the wavelength of the light used for observation. Using these units allows comparison of the properties of different microscopes. [1]

  7. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    2. The comparison microscope 3. The stereoscopic microscope 4. The polarizing microscope 5. The micro spectrophotometer This diversity of the types of microscopes in forensic applications comes mainly from their magnification ranges, which are (1- 1200X), (50 -30,000X) and (500- 250,000X) for the optical microscopy, SEM and TEM respectively. [65]

  8. Multifocal plane microscopy - Wikipedia

    en.wikipedia.org/wiki/Multifocal_plane_microscopy

    The schematic of a multifocal plane microscope. Multifocal plane microscopy (MUM), also known as multiplane microscopy or multifocus microscopy, is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.

  9. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.