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Sodium deoxycholate, the sodium salt of deoxycholic acid, is often used as a biological detergent to lyse cells and solubilise cellular and membrane components. [11] Sodium deoxycholate mixed with phosphatidylcholine , is used in mesotherapy injections to produce lipolysis, and has been used as an alternative to surgical excision in the ...
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Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). [1] [2] This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of ...
1% (w/v) sodium deoxycholate; 0.1% (w/v) SDS; 0.15 M NaCl; 0.01 M sodium phosphate, pH 7.2; 2 mM EDTA; 50 mM sodium fluoride (NaF) 0.2 mM fresh sodium orthovanadate (Na 3 VO 4.2H 2 O, it has phosphatase inhibitor function because it mimics phosphate) 100 U/ml protease inhibitor, such as aprotinin
The original formulation uses sodium deoxycholate to improve solubility. [17] Amphotericin B deoxycholate (ABD) is administered intravenously. [21] As the original formulation of amphotericin, it is often referred to as "conventional" amphotericin. [22]
As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be poured and cooled as soon as possible after addition of the deoxycholate, otherwise ...
Formaldehyde, á-tocopheryl hydrogen succinate, polysorbate 80, sodium deoxycholate, thimerosal, ovalbumin Influenza vaccine ( Fluvirin ) Beta-propiolactone , egg protein, neomycin , nonylphenol ethoxylate, polymyxin , thimerosal (multi-dose containers), thimerosal [ 4 ] (single-dose syringes)
Bile acid synthesis occurs in liver cells, which synthesize primary bile acids (cholic acid and chenodeoxycholic acid in humans) via cytochrome P450-mediated oxidation of cholesterol in a multi-step process.