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A standard ultracentrifuge by manufacturer Beckman Coulter. An ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1 000 000 g (approx. 9 800 km/s²). [1] There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge.
Beckman Coulter, Inc. is a Danaher Corporation company that develops, manufactures, and markets products relevant to biomedical testing. It operates in the industries of diagnostics and life sciences .
[1] [2] [3] During the 1950s, laboratory technicians counted each individual blood cell underneath a microscope. Tedious and inconsistent, this was replaced with the first, very basic hematology analyzer, engineered by Wallace H. Coulter. The early hematology analyzers relied on Coulter's principle (see Coulter counter). However, they have ...
Arnold Orville Beckman (April 10, 1900 – May 18, 2004) was an American chemist, inventor, investor, and philanthropist. While a professor at California Institute of Technology, he founded Beckman Instruments based on his 1934 invention of the pH meter, a device for measuring acidity (and alkalinity), later considered to have "revolutionized the study of chemistry and biology". [1]
There are different types of laboratory centrifuges: Microcentrifuges devices for small tubes from 0.2 ml to 2.0 ml (micro tubes), up to 96 well-plates, compact design, small footprint; up to 30,000 g Clinical centrifuges moderate-speed devices used for clinical applications like blood collection tubes Multipurpose high-speed centrifuges
Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Intact (eukaryotic) cells, macroscopic debris Varies depending on sample Gently lysed cells (e.g. dounce homogenizer) 600 x g 10 min Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Nuclei Cytosol, non-nuclei organelles Supernatant of previous row 15,000 x g 20 min
High-speed or superspeed centrifuges can handle larger sample volumes, from a few tens of millilitres to several litres. Additionally, larger centrifuges can also reach higher angular velocities (around 30,000 rpm). The rotors may come with different adapters to hold various sizes of test tubes, bottles, or microtiter plates.
A tricanter centrifuge operates on a similar principle to decanter centrifuges but instead separates three phases, consisting of a suspended solids phase and two immiscible liquids. [5] Sedimentation of the suspended solids occurs as normal where they accumulate on the wall of the bowl and are conveyed out of the centrifuge.