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Elution principle of column chromatography. In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from a gel electrophoresis or chromatography column.
Another advantage of ion exchange is the predictability of elution patterns (based on the presence of the ionizable group). [12] For example, when cation exchange chromatography is used, certain cations will elute out first and others later. A local charge balance is always maintained.
Ion suppression in LC-MS and LC-MS/MS refers to reduced detector response, or signal:noise as a manifested effect of competition for ionisation efficiency in the ionisation source, between the analyte(s) of interest and other endogenous or exogenous (e.g. plasticisers extracted from plastic tubes, [1] mobile phase additives) species which have not been removed from the sample matrix during ...
An ion observed after this process will consist of the initial neutral molecule [M] with ions added or removed. This is called a quasimolecular ion, for example [M+H] + in the case of an added proton, [M+Na] + in the case of an added sodium ion, or [M-H] − in the case of a removed proton.
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). [2] In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules ...
This can be dependent on the size, charge, or structure of the ions. Common examples of ions that can bind to ion exchangers are: H + and OH − . Singly charged monatomic (i.e., monovalent) ions like Na +, K +, and Cl −. Doubly charged monatomic (i.e., divalent) ions like Ca 2+ and Mg 2+. Polyatomic inorganic ions like SO 2− 4 and PO 3− 4.
ERLIC (eHILIC) separations need not be isocratic, but the net effect is the reduction of the attraction of a particularly strong polar group, which then requires less strong elution conditions, and the enhanced interaction of the remaining polar (opposite charged ionic, or non-ionic) functional groups of the analyte(s).Based on the ERLIC column ...
The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...