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High Resolution Melt (HRM) analysis is a powerful technique in molecular biology for the detection of mutations, polymorphisms and epigenetic differences in double-stranded DNA samples. It was discovered and developed by Idaho Technology and the University of Utah. [1] It has advantages over other genotyping technologies, namely:
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. T m depends on the length of the DNA molecule and its specific ...
DNA nanotechnology is the field that seeks to design nanoscale structures using the molecular recognition properties of DNA molecules. [ 178 ] DNA nanotechnology uses the unique molecular recognition properties of DNA and other nucleic acids to create self-assembling branched DNA complexes with useful properties. [ 179 ]
Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio.
The long concatemers that result are subsequently cleaved between the pac-1 and pac-2 regions of HHV-6's genome by ribozymes when it is packaged into individual virions. [4] A model for HPV16 rolling circle replication. Human Papillomavirus-16 (HPV-16) is another virus that employs rolling replication to produce progeny at a high rate. HPV-16 ...
DNA end resection, also called 5′–3′ degradation, is a biochemical process where the blunt end of a section of double-stranded DNA (dsDNA) is modified by cutting away some nucleotides from the 5' end to produce a 3' single-stranded sequence.
Restriction endonucleases may be found that cleave standard dsDNA (double-stranded DNA), or ssDNA (single-stranded DNA), or even RNA. [citation needed] This discussion is restricted to dsDNA; however, the discussion can be extended to the following: Standard dsDNA; Non-standard DNA; Holliday junctions
Given the difference in widths of the major groove and minor groove, many proteins which bind to DNA do so through the wider major groove. [6] Many double-helical forms are possible; for DNA the three biologically relevant forms are A-DNA , B-DNA , and Z-DNA , while RNA double helices have structures similar to the A form of DNA.