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2. Liquid chromatography–mass spectrometry - Wikipedia

   en.wikipedia.org/wiki/Liquid_chromatography...

   The column is the most important component of the LC system and is designed to withstand the high pressure of the liquid. Conventional LC columns are 100–300 mm long with outer diameter of 6.4 mm (1/4 inch) and internal diameter of 3.0–4.6 mm. For applications involving LC–MS, the length of chromatography columns can be shorter (30–50 ...

3. High-performance liquid chromatography - Wikipedia

   en.wikipedia.org/wiki/High-performance_liquid...

   A modern self-contained HPLC Schematic representation of an HPLC unit (1) solvent reservoirs, (2) solvent degasser, (3) gradient valve, (4) mixing vessel for delivery of the mobile phase, (5) high-pressure pump, (6) switching valve in "inject position", (6') switching valve in "load position", (7) sample injection loop, (8) pre-column (guard column), (9) analytical column, (10) detector (i.e ...

4. Monolithic HPLC column - Wikipedia

   en.wikipedia.org/wiki/Monolithic_HPLC_column

   The basic methods of separation in HPLC rely on a mobile phase (water, organic solvents, etc.) being passed through a stationary phase (particulate silica packings, monoliths, etc.) in a closed environment (column); the differences in reactivity among the solvent of interest and the mobile and stationary phases distinguish compounds from one ...

5. Chromatography - Wikipedia

   en.wikipedia.org/wiki/Chromatography

   The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. The mobile phase ...

6. Charged aerosol detector - Wikipedia

   en.wikipedia.org/wiki/Charged_Aerosol_Detector

   The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.

7. Two-dimensional chromatography - Wikipedia

   en.wikipedia.org/wiki/Two-dimensional_chromatography

   The sample is first subjected to analysis by HPLC and then is subjected to mass analysis. Different types of mass analyzers, ToF, qudrupole, etc., can be used in the MS. [5] Common solvents used in normal or reversed phase LC such as water, acetonitrile, and methanol are all compatible with ESI, yet a LC grade solvent may not be suitable for MS.

8. Direct electron ionization liquid chromatography–mass ...

   en.wikipedia.org/wiki/Direct_electron_ionization...

   The liquid phase from a nano HPLC column is admitted from the capillary column port, where the connection tubing and the nebulizer are first introduced and sealed to prevent vacuum loss. The mechanism is based on the formation of an aerosol in high-vacuum conditions, followed by a quick droplet desolvation and final vaporization of the solute ...

9. Atmospheric-pressure chemical ionization - Wikipedia

   en.wikipedia.org/wiki/Atmospheric-pressure...

   Atmospheric pressure chemical ionization chamber cross section. Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (10 5 Pa), [1] [2] commonly coupled with high-performance liquid chromatography (HPLC). [3]