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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
A close up of an inoculation needle. An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [1] [full citation needed] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [1]
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
This is also known as inoculation, spawning or adding spawn. Its main advantages are to reduce chances of contamination while giving mushrooms a firm beginning. [3] [4] Definition of Spawn: Spawn is a type of medium present in mushroom tissue that propagates the fungus such as Trichoderma which is the root system of mushrooms. [5]
Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
Inoculation paired with other amendments can further improve conditions. Arbuscular mycorrhizal inoculation combined with compost is a common household amendment for personal gardens, agriculture, and nurseries. It has been observed that this pairing can also promote microbial functions in soils that have been affected by mining. [12]
Inoculation is the act of implanting a pathogen or other microbe or virus into a person or other organism. It is a method of artificially inducing immunity against various infectious diseases.